Electrophysiological Measurements

  • Introduction
  • Equipment
  • Prices
  • Contacts
  • Publications

Tampere Facility of Electrophysiological Measurements (MEA laboratory) is situated in BioMediTech (BMT), University of Tampere (UTA). It offers state-of-the-art equipment for electrophysiological recordings, fast fluorescence imaging, and impedance and potential measurements
for life scientists as well as biomedical engineers.

MEA laboratory includes combinable fast fluorescence imaging and patch clamp units, several microelectrode array (MEA) formats, transepithelial electrical resistance (TER, Ussing chamber) and transretinal Electro-retinogram (ERG) system together with additional in-house built measurement systems. The laboratory is equipped with dedicated cell culture facility to enable repeatable measurements in high purity, contrast phase and fluorescence microscopes for additional sample imaging etc. It also contains work bench area where other experimental designs and test experiments can be performed in a designated incubator. Data analysis stations are available for detailed fast imaging and patch clamp analysis.

Current applications areas:

  • patch clamp, fast fluorescence imaging, and MEA with human embryonic and induced pluripotent stem cells derived neural cells and cardiomyocytes
  • patch clamp and fast fluorescence imaging with human pluripotent stem cell derived retinal epithelial pigmented cells
  • fast fluorescence imaging with human retinal pigment epithelial cell line (ARPE-19 cells)
  • MEA with ex vivo retina (mouse/rat origin)

MEA laboratory is equipped and designed in collaboration with BMT (UTA), Department of Electronics and Communications Engineering (Tampere University of Technology, TUT), and Automation Science and Engineering (TUT) under joined BioMediTech Institute. MEA facility provides training for users, organizes imaging related courses and actively participates teaching at UTA and TUT. Users have the possibility to plan and conduct the experiments and data analysis together with the core personnel.


You can view more detailed information by clicking blue equipment names, such as Fast fluorescence imaging unit. The second click will hide the details again.


Fast fluorescence imaging and Patch clamp unit

Fast fluorescence imaging unit

  • Till Photonics Imaging Control Unit

  • Till Photonics Polychrome V
    http://www.till-photonics.com/Products/polychromev.php

  • Olympus IX51

    Features:
    Inverted microscope with Andor iXon 885 camera for fast fluorescence imaging

    Objectives:
    Plan N 4x/0,10
    Plan N 10x/0,25
    LCAch N 20x/0,40
    UApo/340 20x/0,75
    LCAch N 40x/0,55

    Filter sets:
    3 Commonly used filter sets (DAPI, GFP, Cy5.5)


Induced pluripotent stem cell derived cardiomyocytes
labeled with Ca2+-sensitive fluorescent dye,
Heart Group (IBT)
 
Intracellular Ca2+ cycling of a spontaneously beating induced pluripotent
stem cell derived cardiomyocyte, Heart Group (IBT)


Patch Clamp equipment

  • Molecular devices Axopatch 200B amplifier

  • Molecular devices Digidata 1440A Low-Noise data acquisition system

  • L&N Micromanipulators and motorized shifting portal SM5-9

  • NPI SEC-05X amplifiers for dual patch mode

  • Features (for all):
    Patch clamp setup for single cell electrophysiological measurements


Oligodendrocyte precursor cell patched and filled with fluorescent dye, NeuroGroup (IBT)

 

MEA equipment

Multichannel Systems MEA2100

Electrophysiological measurement unit for cells on microelectrode arrays

  • Features:
    • 2 measuring headstages allowing to measure 4x 60-channel MEAs simultaneously or 2x 60-channel MEAs and a 120-channel MEA simultaneously
    • Built in system for electrical stimulation of the cells
    • Phase contrast and DIC microscopy options available during measurements
    • Heater unit included in the systems to allow temperature control and monitoring
    • MC_Rack software for data acquisition
    • http://www.multichannelsystems.com/systems/mea2100-2x60-2-system

Arraywide neuronal network activity measured on MEA2100, NeuroGroup (IBT)


Multichannel Systems USB-MEA1060

  • Features:
    • Portable microelectrode array system for electrophysiological measurements
    • Heater unit included in the systems to allow temperature control and monitoring
    • MC_Rack software for data acquisition

Bursting neuronal cells measured with microelectrode array, NeuroGroup (IBT)


Multichannel Systems MEA1060-Inv

  • Features:
    • Microelectrode array system for electrophysiological measurements
    • Perfusion element to use perforated MEAs (pMEA) for tissue slice/retinal recordings
    • Stimulus generator for electrical and/or light stimulation of the cells
    • Dark room built for light sensitive experimentsHeater unit included in the systems to allow temperature control and monitoring
    • MC_Rack software for data acquisition
    • http://www.multichannelsystems.com/products/mea1060-inv-bc

Retina MEA recordings, Electronics and Communications Engineering (TUT)


Ussing chamber system

Physiologic Instruments 8-chamber EM-CSYS-8 Easy Mount Ussing chamber system

  • Features:
    • Ussing chamber system for the measurements of epithelial transport and electrical responses; allows simultaneous measurements from 8 samples
    • Multichannel voltage/current clamp equipment included in the system for electrical recordings

 

Conventional bright field microscopes

Zeiss Axio Vert A1

  • Features:
    • Inverted microscope with DIC option
  •  
  • Objectives:
    • N-ACHROPlan 5x/0,15
    • A-Plan 10x/0,25
    • LD-A 20x/0,35
    • LD-A 40x/0,55

 

Cell Culturing facilities

CO2 Incubators and Laminar flow cabinets

4 CO2 incubators for cell culturing and 2 Kojair laminar flow cabinets

  • Features:
    • Laminar flow to protect samples from particle contamination during preparation and CO2 incubators for providing long term cell culture environment



Prices will be defined based on customer needs. Please inquire!



Picture: Sami Helenius

Reservations, equipment details:

Juha Heikkilä, B.Sc.
juha.heikkila(at)uta.fi
Tel: +358 40 190 1798
Room: ARVO D443


User prices:

Susanna Narkilahti, Ph.D., Adj. Prof.
susanna.narkilahti(at)uta.fi
Tel: +358 40 708 5113
Room: ARVO D428


Picture: Sami Helenius


Publications before 2013

Ryynänen, T., Ylä-Outinen, L., Narkilahti, S., Tanskanen, J.M.A., Hyttinen, J., Hämäläinen, J., Leskelä, M., Lekkala, J.
ALD IrOx Thin Film as Microelectrode Coating in Stem Cell Application
Journal of Vacuum Science and Technology A 30(4), Jul/Aug 2012

Äänismaa R, Hautala J, Vuorinen A, Miettinen S, Narkilahti S
Human dental pulp stem cells differentiate into neural precursors but not into mature functional neurons
Stem Cell Discovery. 2012, 2(3):85-91

Kapucu FE, Tanskanen JMA, Mikkonen JE, Ylä-Outinen L, Narkilahti S and Hyttinen JAK
Burst analysis tool for developing neuronal networks exhibiting highly varying action potential dynamics
Front. Comput. Neurosci. 2012, 6:38. doi: 10.3389/fncom.2012.00038

Kujala K, Paavola J, Lahti A, Larsson K, Pekkanen-Mattila M, Viitasalo M, Lahtinen AM, Toivonen L, Kontula K, Swan H, Laine M, Silvennoinen O, Aalto-Setälä K
Cell Model of Catecholaminergic Polymorphic Ventricular Tachycardia Reveals Early and Delayed Afterdepolarizations
PLoS ONE 2012;7(9):e44660

Kujala K, Ahola A, Pekkanen-Mattila M, Ikonen L, Kerkelä E, Hyttinen J and Aalto-Setälä K
Electrical Field Stimulation with a Novel Platform: Effect on Cardiomyocyte Gene Expression but not on Orientation
International Journal of Biomedical Science 2012, 8(2), 109-120

Ylä-Outinen L, Joki T, Varjola M, Skottman H, Narkilahti S
Three dimensional growth matrix for human embryonic stem cell-derived neuronal cells
The Journal of Tissue Engineering and Regenerative Medicine. 2012 May 18

Kreutzer J, Ylä-Outinen L, Kärnä P, Kaarela T, Skottman H, Mikkonen J, Narkilahti S, Kallio P
Structured PDMS chambers for enhanced human neuronal cell activity on MEA platforms
Journal of Bionic Engineering 2012, 9(1):1-10

Lahti AL, Kujala VJ, Chapman H, Koivisto AP, Pekkanen-Mattila M, Kerkelä E, Hyttinen J, Kontula K, Swan H, Conklin BR, Yamanaka S, Silvennoinen O, Aalto-Setälä K
Model for long QT syndrome type 2 using human iPS cells demonstrates arrhythmogenic characteristics in cell culture
Dis Model Mech. 2012 Mar;5(2):220-30

Kujala VJ, Jimenez ZC, Väisänen J, Tanskanen JM, Kerkelä E, Hyttinen J, Aalto-Setälä K
Averaging in vitro cardiac field potential recordings obtained with microelectrode arrays
Comput Methods Programs Biomed. 2011 Nov;104(2):199-205

Ryynänen T, Kujala V, Ylä-Outinen L, Korhonen I, Tanskanen J, Aalto-Setälä K, Hyttinen J, Kerkelä E, Narkilahti S, Lekkala J
All Titanium Microelectrode Array for Field Potential Measurements from Neurons and Cardiomyocytes - a Feasibility Study
Micromachines 2, 394-409 (2011)

Lappalainen RS, Salomäki M, Ylä-Outinen L, Heikkilä TJ, Hyttinen JAK, Pihlajamäki H, Suuronen R, Skottman H, Narkilahti S
Similarly derived and cultured hESC lines show variation in their developmental potential towards neuronal cells in long-time culture
Regenerative Medicine, 2010 Sep;5(5):749-62

Ylä-Outinen L, Heikkilä J, Skottman H, Suuronen R, Äänismaa R, Narkilahti S
Human cell-based micro electrode array platform for studying neurotoxicity
Frontiers in Neuroengineering, 2010, 3(111):1-9

Heikkilä T, Ylä-Outinen L, Tanskanen J, Lappalainen R, Skottman H, Suuronen R, Hyttinen J, Narkilahti S
Human embryonic stem cell-derived neuronal cells form spontaneously active neuronal networks in vitro
Exp Neurol. 2009 Jul;218(1):109-16

Pekkanen-Mattila M, Kerkelä E, Tanskanen JM, Pietilä M, Pelto-Huikko M, Hyttinen J, Skottman H, Suuronen R, Aalto-Setälä K
Substantial variation in the cardiac differentiation of human embryonic stem cell lines derived and propagated under the same conditions - a comparison of multiple cell lines
Ann Med. 2009;41(5):360-70