Multimodal Tomographic Microscopy

  • Introduction
  • Equipment
  • Prices
  • Contacts

Multimodal Tomographic Microscopy facility offers real 3D imaging of materials and tissues in micrometer resolution. It is situated in Computational Biophysics and Imaging Group (CBIG) in Tampere University of Technology Finn Medi 1. It offers 2 different tomographic imaging systems: an X-ray microtomography (microCT) and an optical projection tomography (OPT) microscopes. In addition to the imaging systems the facility has state of the art data processing and analysis workstation available with visualization and 3D-image quantification possibilities with Avizo Fire (www.vsg3d.com/avizo/fire).


X-ray microtomography

Our Zeiss Xradia microCT can be used to determine morphological parameters of materials, biomaterials and biological samples with resolution down to 1 micron. On the other hand, it can accommodate large samples in cm and dm scale at the expense of resolution.

X-ray computed tomography, is a non-destructive means to create cross-sectional images of a three-dimensional object. The device takes hundreds of X-ray images around the object and calculates its internal 3D structure based on the density distribution of the material. Thus the contrast in X-ray-CT images is based on the density variation within the object and can be enhanced with various non-specific and specific contrast agents. Biological samples are often fixed because the imaging may take a few hours and X-rays can harm living cells.


OPT

Optical projection tomography (OPT) is the optical equivalent of Micro-CT, that produces high-resolution (5 microns) 3-Dimensional image reconstructions of both fluorescently stained and non-fluorescent (transmission) specimens ranging in size from 1mm to 15mm. In OPT a suspended specimen, in an index-matching liquid, is rotated through a series of angular positions, and an image is captured at each orientation with a camera. Images collected in each orientation can be reconstructed similar way as in X-ray CT and the 3D structure of the sample can be recovered.

The OPT was built by our researchers so it is very flexible in what concerns the light sources, filters and lens that can be adapted to different types of samples.

The system is being used for imaging transparent biomaterials with/without cells but usual applications include visualization of anatomy (phenotyping), gene expression (in situ hybridization), protein distribution (immunohistochemistry or GFP expression), transgenic visualization (LacZ). Typical specimens include mouse and chicken embryos, mouse/rat tissue and organs, zebrafish (5 days and older), drosophila, plants etc.



XRadia MicroXCT-400

  • X-ray source:
    Hamamatsu L8121-03

  • Detector:
    Optical objectives (0.4x, 1x, 4x, 10x, 20x) with scintillators

  • Filters:
    6 low-energy filters, 6 high-energy filters

  • Camera:
    Andor iKon L DW936N-BV

  • Max. field of view:
    about 70 mm

  • Resolution varies depending on the field of view:
    approximately 1-70 µm

  • Max. sample weight:
    15 kg

Optical Projection Tomography

  • Light source (bright-field mode):
    White LED spot light

  • Light source (fluorescent mode):
    Different LED sources: 365, 470, 590, and 660 nm

  • Detector:
    Optical objectives (5x, 10x)

  • Filters:
    Filter for fluerescense detection: 458, 578, 623, and 716 nm

  • Camera:
    Hamamatsu Orca-Flash 4.0

  • Max. field of view:
    about 3 mm

  • Resolution varies depending on the field of view:
    approximately 10 µm

  • Max. sample weight:
    500 g


Prices will be defined based on customer needs. Please inquire!


Facility director:

Professor Jari Hyttinen
jari.hyttinen(at)tut.fi
Tel: +358 40 849 0020