Zebrafish Laboratory

  • Introduction
  • Equipment
  • Prices
  • Contacts
  • Publications
  • Genetic Screen

Zebrafish (Danio rerio) are small freshwater fish popular in home aquaria. In the last decade interest in using zebrafish models in genetic and biomedical research has increased greatly. Zebrafish provide a vertebrate model in which many advantages of lower invertebrate model organisms are combined to the advantages of mammalian research models:

  • Embryonic development ex utero
  • Transparency of embryos
  • Rapid embryonic development
  • Fast progeny production
  • Genetically traceable
  • Easy genetic manipulation
  • Suitability for large scale genetic screens
  • Anatomical and physiological similarities with mammals
  • Several existing human disease models

Animal welfare

Keeping and raising of zebrafish stocks in our facility is performed under permission of the State Provincial Office of Western Finland. Care of animals is done following the Finnish Laboratory Animal Welfare Act 62/2006 and the Laboratory Animal Welfare Ordinance 36/2006.

Services

  • Housing of zebrafish
  • Morpholino-injections
  • F3 homozygous mutant carrier families for genetic screening

Our facility serves all scientists using zebrafish as a research model system. The new facility is capable of housing up to 50 000 adult zebrafish. It is composed of a laboratory room, a microscope room for fluorenscence microscopy and imaging, one large fish room with two separate systems, and two quarantine rooms with stand-alone units. The zebrafish housing system was designed and installed by Aquatic Habitats. The facility is equipped with a reverse osmosis water purification system, and an Aquatic Habitats YSI system which automatically monitors pH, conductivity, temperature and dissolved oxygen in the fish water.

The system is capable of maintaining consistent pH and conductivity by dosing salt and sodium bicarbonate solutions automatically as necessary. The system water recirculates through several filtration units, including mechanical, biological and chemical filters, as well as an ultraviolet sterilizer. The systems are connected to a Sensaphone paging system 24 h/day.



The facility is equipped with:

  • 2 WPI Pneumatic Picopump microinjectors and Narishige micromanipulators

  • Nikon SMZ645 stereomicroscope

  • Nikon SMZ800 stereomicroscope

  • Zeiss Lumar V1.1 fluorescence stereomicroscope

  • Sutter P-97 capillary needle puller


Service Price
Zebrafish facility user fee 1000 €/year/research group
Training 50 €/h
Tank fee (1,5 l) 50 €/year
Tank fee (3 l) 100 €/year
Tank fee (10 l) 150 €/year
100 embryos, wild type 50 €
100 embryos, mutant/transgenic 100 €
Technical assistance 30 €/h
Morpholino-injected embryos 100 €/100 embryos
Genotyping 5 €/1 adult zebrafish
Adult zebrafish, wild type < 100 fish: 6 €/fish
≥ 100 fish: 4 €/fish
Adult zebrafish, mutant/transgenic 5-30 €
Fixed embryos/larvae 1-7 dpf 50 €/50 embryos


Zebrafish facility:
Zflab(at)uta.fi
Tel: +358 50 318 3864

Director:
Mataleena Parikka, D.D.S., Ph.D.
mataleena.parikka(at)uta.fi
Room: ARVO D432
Tel: +358 40 735 5052

Facility Manager:
Leena Mäkinen
leena.makinen(at)uta.fi
Room: ARVO F324
Tel: +358 40 190 9728


2016

Myllymäki H, Bäuerlein CA, Rämet M
The Zebrafish Breathes New Life into the Study of Tuberculosis.
Front Immunol ;2016. PMID: 27242801

Oksanen KE, Myllymäki H, Ahava MJ, Mäkinen L, Parikka M, Rämet M
DNA vaccination boosts Bacillus Calmette-Guérin protection against mycobacterial infection in zebrafish.
Dev Comp Immunol ;54(1)89-96, 2016. PMID: 26363085

2015

Aspatwar A, Tolvanen ME, Ojanen MJ, Barker HR, Saralahti AK, Bäuerlein CA, Ortutay C, Pan P, Kuuslahti M, Parikka M, Rämet M, Parkkila S
Inactivation of ca10a and ca10b Genes Leads to Abnormal Embryonic Development and Alters Movement Pattern in Zebrafish
PLoS One. 2015 Jul 28;10(7):e0134263. doi: 10.1371/journal.pone.0134263. eCollection 2015

Saralahti A, Rämet M
Zebrafish and Streptococcal Infections
Scand J Immunol. 2015 Sep;82(3):174-83. doi: 10.1111/sji.12320

Myllymäki H, Niskanen M, Oksanen KE, Rämet M
Animal models in tuberculosis research - where is the beef?
Expert Opin Drug Discov. 2015;10(8):871-83. doi: 10.1517/17460441.2015.1049529. Epub 2015 Jun 13

Ojanen MJ, Turpeinen H, Cordova ZM, Hammarén MM, Harjula SK, Parikka M, Rämet M, Pesu M
The proprotein convertase subtilisin/kexin furinA regulates zebrafish host response against Mycobacterium marinum
Infect Immun. 2015 Apr;83(4):1431-42. doi: 10.1128/IAI.03135-14. Epub 2015 Jan 26


2014

Hammarén MM, Oksanen KE, Nisula HM, Luukinen BV, Pesu M, Rämet M, Parikka M
Adequate Th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish
PLoS Pathog. 2014 Jun 26;10(6):e1004190. doi: 10.1371/journal.ppat.1004190. eCollection 2014 Jun

Saralahti A, Piippo H, Parikka M, Henriques-Normark B, Rämet M, Rounioja S
Adult zebrafish model for pneumococcal pathogenesis
Dev Comp Immunol. 2014, 42(2):345-53


2013

Oksanen KE, Halfpenny NJ, Sherwood E, Harjula SK, Hammarén MM, Ahava MJ, Pajula ET, Lahtinen MJ, Parikka M & Rämet M
An adult zebrafish model for preclinical tuberculosis vaccine development
Vaccine,31:5202-9

Taskinen B, Zmurko J, Ojanen M, Kukkurainen S, Parthiban M, Määttä JAE, Leppiniemi J, Jänis J, Parikka M, Turpeinen H, Rämet M, Pesu M, Johnson MS, Kulomaa MS, Airenne TT & Hytönen VP
Zebavidin - An avidin-like protein from zebrafish
PLoS ONE,8(10): e77207

Turpeinen H, Oksanen A, Kivinen V, Oksanen A, Kukkurainen S, Uusimäki A, Rämet M, Parikka M, Hytönen V, Nykter M & Pesu M
Proprotein Convertase Subtilisin/Kexin Type 7 (PCSK7) is essential for the zebrafish development and bioavailability of Transforming Growth Factor Beta 1a (TGFβ1a)
Journal of Biological Chemistry,288:36610-23

Aspatwar A, Tolvanen M, Jokitalo E, Parikka M, Ortutay C, Harjula SK, Rämet M, Vihinen M & Parkkila S
Altered cerebellum development and motor dysfunction in CARP VIII morphant zebrafish embryos
Hum Mol Genet. 2013 Feb 1;22(3):417-32


2012

Teittinen K, Parikka M, Latvala A, Junttila S, Laiho A, Korkeamäki H, Gröngroos T, Kurppa K, Turpeinen H, Pesu M, Gyenesei A, Rämet M & Lohi O
Knockdown of SAP30L disrupts early heart development and function in zebrafish
Journal of Cellular Biochemistry, 2012, 113:3843-3852

Lohi O, Parikka M & Rämet M
The zebrafish as a model for paediatric diseases
Acta Paediatrica. Epub Aug 24, 2012, doi: 10.1111/j.1651-2227.2012.02835.x

Parikka M, Vuoksio M, Harjula SK, Halfpenny N, Oksanen K, Lahtinen MJ, Pajula E, Iivanainen A, Pesu M & Rämet M
Mycobacterium marinum causes a latent infection that can be reactivated by gamma irradiation in adult zebrafish
PLoS Pathogens, 2012, 8:e1002944

Patterson H, Saralahti A, Parikka M, Dramsi S, Trieu-Cuot P, Poyart C, Rounioja S & Rämet M
Adult zebrafish model of bacterial meningitis in Streptococcus agalactiae infection
Developmental and Comparative Immunology, Epub Aug 4, 2012

Teittinen K, Grönroos T, Parikka M, Rämet M & Lohi O
The zebrafish as a tool in leukemia research
Leukemia Research, 2012, 36:1082-1088

Rounioja S, Saralahti A, Rantala L, Parikka M, Henriques-Normark B, Silvennoinen O, Rämet M
Defense of zebrafish embryos against Streptococcus pneumoniae infection is dependent on the phagocytic activity of leukocytes
Developmental and Comparative Immunology, 2012, 36(2):342-348


2011

Ulvila J, Vanha-aho LM, Kleino A, Vähä-Mäkilä M, Vuoksio M, Eskelinen S, Hultmark D, Kocks C, Hallman M, Parikka M & Rämet M
Cofilin regulator 14-3-3-zeta is an evolutionarily conserved protein required for phagocytosis and microbial resistance
Journal of Leukocyte Biology, 2011,89(5):649-659


2010

Hyvärinen J, Parikka M, Sormunen R, Rämet M, Tryggvason K, Kivirikko KI, Myllyharju J & Koivunen P
Deficiency of a transmembrane prolyl 4-hydroxylase in the zebrafish leads to basement membrane defects and compromized kidney function
Journal of Biological Chemistry, 2010, 285(53):42023-42032

Valanne S, Myllymäki H, Kallio J, Schmid M, Kleino A, Murumägi A, Airaksinen L, Kotipelto T, Kaustio M, Ulvila J, Esfahani S, Engström Y, Silvennoinen O, Hultmark D, Parikka M & Rämet M
Genome-wide RNAi in Drosophila cells identifies G protein-coupled receptor kinase 2 as an evolutionary conserved regulator of the NF-kB signaling
Journal of Immunology, 2010, 184:6188-6198



Genetic screen

As a tool for screening to identify new genes and genetic networks, we have established a collection of zebrafish lines carrying random mutations in their genomes. The lines have been created by using gene-breaking transposon as insertional mutagen (Clark et al., 2011, Nature Methods).

The collection of mutant lines is now available to the research community for screening of phenotypes of interest. We provide F2 and F3 mutant families, either embryos or adults. New lines are continuously being added to the collection, within the space limitations of our zebrafish laboratory.

For further questions or requests, please contact us.

(September 12th, 2013)